ATX-304 CAS # 1261289-04-6

ATX-304 (O-304) is a synthetic small-molecule research compound engineered as a potent, pan-activator of AMP-activated protein kinase (AMPK) — the master regulator of cellular energy homeostasis. Characterized by a substituted benzothiadiazole core (CAS 1261289-04-6), it is designed for high-precision investigation of AMPK-dependent signaling, intracellular energy metabolism, and kinase-ligand binding dynamics in laboratory settings.

Originally developed by researchers at Umeå University and Betagenon AB in Sweden, ATX-304 has advanced to Phase 2 clinical investigation in obesity and cardiometabolic disease — making it one of the most clinically relevant AMPK-targeted research compounds available today.

Technical Specifications

• CAS Number: 1261289-04-6
• Chemical Formula: C₁₆H₁₁Cl₂N₃O₂S
• Molecular Weight: 380.2 g/mol
• Chemical Class: AMPK Activator (Benzothiadiazole derivative)
• Assay Purity: >98% (HPLC verified)
• Appearance: White powder

Mechanism of Action
ATX-304 acts as a selective allosteric activator across all three AMPK heterotrimeric complexes. Its benzothiadiazole scaffold binds to the catalytic domain of AMPK, stabilizing the active enzyme conformation and driving downstream phosphorylation of key metabolic targets including ACC, HMGCR, and TSC2. This allows researchers to study glucose and lipid oxidation regulation, mitochondrial biogenesis signaling, and energy-sensing pathway dynamics in isolated cellular systems.

Research Applications

• Enzyme Binding Assays — quantifying ATX-304/AMPK binding affinity in cell-free preparations via competitive displacement
• Kinase Activity Studies — fluorescence-based measurement of AMPK catalytic efficiency modulation
• Metabolic Signaling Research — investigating downstream phosphorylation cascades in isolated metabolic pathway models
• Structural Biology — mapping chlorine-substituted heterocycle interactions within kinase catalytic sites

Storage & Handling

• Powder: Store at -20°C, protected from light, in sealed amber vials
• Capsules: Room temperature in original packaging
• Reconstituted solutions: Use within 30 days at 4°C

Disclaimer: ATX-304 is sold exclusively for laboratory research use. It is not an FDA-approved drug, dietary supplement, or medical treatment, and is strictly prohibited for human or animal consumption. For research purposes only.